1RZY

Crystal structure of rabbit Hint complexed with N-ethylsulfamoyladenosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-10-26
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	39.888, 39.888, 142.286
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.000 - 1.800
R-factor	0.217
Rwork	0.216
R-free	0.24200
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.013
RMSD bond angle	1.400 *
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.065 *	0.143 *
Total number of observations	95355 *
Number of reflections	10698
<I/σ(I)>	9.2	5.1
Completeness [%]	93.9	88.3
Redundancy	8.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	298	Brenner, C., (1997) Nat.Struct.Biol., 4, 231. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG8000	30 (%)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)