1RZV
Crystal structure of the glycogen synthase from Agrobacterium tumefaciens (non-complexed form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-11-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.271, 87.289, 88.492 |
| Unit cell angles | 90.00, 100.29, 90.00 |
Refinement procedure
| Resolution | 29.970 - 2.300 |
| R-factor | 0.2245 |
| Rwork | 0.204 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM (6.2.0) |
| Data scaling software | SCALA (2.7.5) |
| Phasing software | SnB (0.95) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.970 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.257 |
| Number of reflections | 88607 | |
| <I/σ(I)> | 11.4 | 5.5 |
| Completeness [%] | 98.2 | 85.9 |
| Redundancy | 6.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 4000, isopropanol, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
