1RZQ

Crystal Structure of C-Terminal Despentapeptide Nitrite Reductase from Achromobacter Cycloclastes at pH5.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	CONVENTIONAL Cu
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	99.020, 117.440, 122.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.181
R-free	0.20800
Structure solution method	ISOMORPHOUS DIFFERENCE FOURIER SYNTHESIS
Starting model (for MR)	1rzp
RMSD bond length	0.005
RMSD bond angle	1.570
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.130	0.586
Number of reflections	73098
<I/σ(I)>	13.4	3
Completeness [%]	99.8	99.3
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	1.4M ammonium sulfate, 0.1M acetate acid pH 5.0, 0.2M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K