1RZP

Crystal Structure of C-Terminal Despentapeptide Nitrite Reductase from Achromobacter Cycloclastes at pH6.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	98.880, 117.690, 123.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
Rwork	0.179
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nia
RMSD bond length	0.005
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.119	0.529
Number of reflections	112590
<I/σ(I)>	14.5	2.9
Completeness [%]	99.5	98.5
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	1.6M ammonium sulfate, 0.1M Mes, 0.2M sodium chloride, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K