1RZO
Agglutinin from Ricinus communis with galactoaza
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.801 |
| Spacegroup name | P 32 |
| Unit cell lengths | 97.630, 97.630, 207.827 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.110 - 2.630 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aai |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.820 |
| High resolution limit [Å] | 2.600 | 2.630 |
| Rmerge | 0.060 | 0.260 |
| Number of reflections | 67524 | |
| <I/σ(I)> | 14.74 | 4.87 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 3.86 | 3.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 1,7 amonium sulphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
