1RZL

RICE NONSPECIFIC LIPID TRANSFER PROTEIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	290
Detector technology	DIFFRACTOMETER
Collection date	1992-05-05
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 43 21 2
Unit cell lengths	37.930, 37.930, 97.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.600
R-factor	0.186
Rwork	0.186
R-free	0.23500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mzl
RMSD bond length	0.017
RMSD bond angle	21.600 *
Data reduction software	MADNES
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.670
High resolution limit [Å]	1.600	1.600
Rmerge	0.055
Total number of observations	33853 *
Number of reflections	9634 *
Completeness [%]	94.4 *	82.7 *
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.7 *		pH 7.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	CAPS	50 (mM)
2	1	drop	ammonium sulfate	1.8 (M)
3	1	drop	protein	18 (mg/ml)
4	1	reservoir	ammonium sulfate	3.0 (M)
5	1	reservoir	CAPS	50 (mM)