1RZ9

Crystal Structure of AAV Rep complexed with the Rep-binding sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-12
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	78.671, 131.706, 82.164
Unit cell angles	90.00, 112.68, 90.00

Refinement procedure

Resolution	20.000 * - 3.100
R-factor	0.286
Rwork	0.286
R-free	0.31600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m55
RMSD bond length	0.010
RMSD bond angle	1.330 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.100 *	3.000
Total number of observations	72828 *
Number of reflections	23005 *
<I/σ(I)>	6.2	3
Completeness [%]	87.8 *	74.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5 *	4 *	VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	20 (mM)	pH7.5
2	1	reservoir		0.12 (M)
3	1	drop	PEG3000	20 (%(w/v))
4	1	drop	sodium citrate	0.1 (M)	pH5.5