1RZ4

Crystal Structure of Human eIF3k

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-10
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	83.074, 44.671, 55.524
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.208
Rwork	0.187
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.102
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.6M ammonium sulfate, 0.1M Hepes, 0.1M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K