1RYZ
Uridine Phosphorylase from Salmonella typhimurium. Crystal Structure at 2.9 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 61 |
| Unit cell lengths | 92.260, 92.260, 267.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.460 - 2.900 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k3f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.154 | 0.338 |
| Number of reflections | 26462 | |
| <I/σ(I)> | 6.89 | 3.05 |
| Completeness [%] | 93.0 | 75.9 |
| Redundancy | 2.84 | 2.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 0.1M Na Acetate trihydrate, 10% PEG 8000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
