1RYY
Acetobacter turbidans alpha-amino acid ester hydrolase Y206A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.649, 177.513, 169.967 |
| Unit cell angles | 90.00, 91.03, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.21104 |
| Rwork | 0.210 |
| R-free | 0.23587 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.271 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 134932 | |
| <I/σ(I)> | 17.2 | 6.9 |
| Completeness [%] | 99.4 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | Sodium acetate, PEG 4000, ammonium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
