1RYW
C115S MurA liganded with reaction products
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 85 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-14 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.867, 153.729, 135.089 |
| Unit cell angles | 90.00, 104.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ejc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.320 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 140111 | |
| <I/σ(I)> | 12 | 3 |
| Completeness [%] | 98.9 | 97.3 |
| Redundancy | 6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | PEG 20,000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
