Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-06 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.519, 101.556, 52.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.340 - 2.280 |
| R-factor | 0.253 |
| Rwork | 0.253 |
| R-free | 0.28200 * |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.720 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.340 | |
| High resolution limit [Å] | 2.280 | 2.280 * |
| Rmerge | 0.070 * | 0.554 * |
| Number of reflections | 24302 | |
| Completeness [%] | 98.9 * | 99 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | PEG 3350, CA(AC)2 , pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | CA(AC)2 | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | PEG 3350 | ||
| 5 | 1 | 2 | CA(AC)2 | ||
| 6 | 1 | 2 | H2O |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 15 (%) | |
| 2 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | glycerol | 2.5 (%) | |
| 4 | 1 | drop | Dpo4 | 6 (mg/ml) | |
| 5 | 1 | drop | HEPES | 20 (mM) | pH7.0 |
| 6 | 1 | drop | 5 (mM) | ||
| 7 | 1 | drop | 150 (mM) |
