1RYQ
Putative DNA-directed RNA polymerase, subunit e'' from Pyrococcus Furiosus Pfu-263306-001
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.534, 45.534, 50.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.530 - 1.380 |
| R-factor | 0.19374 |
| Rwork | 0.193 |
| R-free | 0.20435 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.337 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (refmac_5.1.24 24/04/2001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.062 | 0.207 |
| Number of reflections | 12502 | |
| Completeness [%] | 79.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 297 | 100mM sodium citrate, 25% PEG 3000, pH 6.6, modified batch crystallization, temperature 297K, pH 6.60 |
