1RYL
The Crystal Structure of a Protein of Unknown Function YfbM from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-12 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795,0.9797,0.94656 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.848, 60.455, 57.931 |
| Unit cell angles | 90.00, 114.62, 90.00 |
Refinement procedure
| Resolution | 30.230 - 1.600 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.372 |
| Number of reflections | 78702 | |
| <I/σ(I)> | 15.85 | 1.223 |
| Completeness [%] | 93.2 | 55.5 |
| Redundancy | 2.37 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2 M MgCl2, 0.1 M HEPES, 30%PEG3350, 8% Ethanol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
