1RYI

STRUCTURE OF GLYCINE OXIDASE WITH BOUND INHIBITOR GLYCOLATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-28
Detector	MARRESEARCH
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	73.713, 218.765, 217.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 1.800
R-factor	0.17881
Rwork	0.177
R-free	0.21399
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.320
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	REFMAC (5.1.25)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	159578
Completeness [%]		96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	291	10% w/v PEG 1000, 100 mM Imidazole, 200 mM Ca-Acetate, 30 mM Sodium-Glycolate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K