1RY8
Prostaglandin F synthase complexed with NADPH and rutin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2003-08-13 |
Detector | SBC-3 |
Wavelength(s) | 1.02 |
Spacegroup name | P 1 |
Unit cell lengths | 47.060, 49.370, 83.590 |
Unit cell angles | 73.90, 86.30, 69.90 |
Refinement procedure
Resolution | 10.000 - 1.690 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ryo |
RMSD bond length | 0.060 |
RMSD bond angle | 1.500 |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Number of reflections | 72690 | |
Completeness [%] | 92.9 | 90.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |