1RXX
Structure of arginine deiminase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98400, 0.96788, 0.97925, 0.97939 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 114.900, 114.900, 300.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.450 |
| Rwork | 0.198 |
| R-free | 0.26400 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.096 | 0.416 |
| Total number of observations | 829494 * | |
| Number of reflections | 74771 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | 33% MPD (2-methyl-2,4-pentanediol), 6% PEG 3350, 0.1 M Tris-HCl (pH 7.6), and 4% acetone, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | reservoir | MPD | 33 (%) | |
| 4 | 1 | reservoir | PEG3350 | 6 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.6 |
| 6 | 1 | reservoir | acetone | 4 (%) |
