1RXV
Crystal Structure of A. Fulgidus FEN-1 bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.843, 86.843, 250.519 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.276 |
| Rwork | 0.274 |
| R-free | 0.23800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model built from 2.5A resolution experimental map. Phases for this map were calculated from a four-wavelength multiple anomalous dispersion experiment. |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.290 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 33196 | |
| Completeness [%] | 97.3 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.8 | 21 * | MPEG 5000, sodium acetate, ethylene glycol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPEG 5000 | ||
| 2 | 1 | 1 | sodium acetate | ||
| 3 | 1 | 1 | ethylene glycol |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | MPEG5000 | 5 (%) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.8 |
| 4 | 1 | reservoir | ethylene glycol | 5 (%) |
