1RWT

Crystal Structure of Spinach Major Light-harvesting complex at 2.72 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-11-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	261.787, 261.787, 660.498
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.720
R-factor	0.194
Rwork	0.194
R-free	0.22100
Structure solution method	SIR in combination with real-space averaging refinement and extension
RMSD bond length	0.012
RMSD bond angle	2.300
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.082	0.368
Number of reflections	191660
<I/σ(I)>	14.4	2.5
Completeness [%]	90.8	79.6
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	Citrate trisodium, beta-nonyl-glucoside, deoxy-bigchap, DGDG, Hepes, pH 7.5, VAPOR DIFFUSION, temperature 291K
1	VAPOR DIFFUSION	7.5	291	Citrate trisodium, beta-nonyl-glucoside, deoxy-bigchap, DGDG, Hepes, pH 7.5, VAPOR DIFFUSION, temperature 291K