1RWJ
c7-type three-heme cytochrome domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-19 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.03320, 1.73769, 1.74044, 1.69031, 1.78780 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 43.800, 43.800, 123.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.280 - 1.700 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.21800 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 3.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.274 |
| Number of reflections | 25221 | |
| <I/σ(I)> | 61 | 13 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 10 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.4 M sodium malonate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
