1RW9
Crystal structure of the Arthrobacter aurescens chondroitin AC lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.569, 86.455, 80.483 |
| Unit cell angles | 90.00, 106.90, 90.00 |
Refinement procedure
| Resolution | 36.040 - 1.350 |
| R-factor | 0.1307 |
| Rwork | 0.130 |
| R-free | 0.17503 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Hg-derivative of the same protein |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.973 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 178066 | |
| <I/σ(I)> | 8.6 | 3.4 |
| Completeness [%] | 96.4 | 93.4 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | PEG 8000, ammonium acetate, phosphate buffer, glycerol, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
