1RW0
Crystal structure of protein yfiH from Salmonella enterica serovar Typhi, Pfam DUF152
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | .979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.330, 62.123, 77.745 |
| Unit cell angles | 90.00, 110.22, 90.00 |
Refinement procedure
| Resolution | 39.020 - 2.000 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rv9 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.088 | 0.242 |
| Number of reflections | 31438 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 2.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | Ammonium Acetate, Sodium Acetate trihydrate,PEG4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 20K |
