1RVX

1934 H1 Hemagglutinin in complex with LSTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-26
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	C 1 2 1
Unit cell lengths	227.878, 131.344, 174.708
Unit cell angles	90.00, 110.09, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.227
Rwork	0.227
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ru7
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.071	0.528
Number of reflections	216839
<I/σ(I)>	17.8	2
Completeness [%]	88.8	50.6
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	PEG3350, TrisHCl, KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K