1RVK

Crystal structure of enolase AGR_L_2751 from Agrobacterium Tumefaciens

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	110
Detector technology	CCD
Collection date	2003-11-20
Detector	MARRESEARCH
Wavelength(s)	0.97911, 0.97934, 0.97166
Spacegroup name	I 4 2 2
Unit cell lengths	118.733, 118.733, 133.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.700
R-factor	0.195
Rwork	0.193
R-free	0.21200
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.320
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.085	0.570
Number of reflections	50443
<I/σ(I)>	24.6	4.2
Completeness [%]	96.4	91.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	sodium chloride, hepes, pH 7.5, VAPOR DIFFUSION, temperature 293K