1RVG

crystal structure of class II fructose-bisphosphate aldolase from Thermus aquaticus in complex with Y

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Wavelength(s)	1.105
Spacegroup name	P 1 2 1
Unit cell lengths	99.860, 57.550, 138.617
Unit cell angles	90.00, 90.26, 90.00

Refinement procedure

Resolution	40.600 * - 2.000
R-factor	0.225
Rwork	0.225
R-free	0.25900
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	22.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	FFT
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.000
Rmerge	0.067 *	0.400 *
Total number of observations	912165 *
Number of reflections	99328
Completeness [%]	92.8	92.9 *
Redundancy	9.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	295 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1.75 (mg/ml)
2	1	drop	sucrose monolaurate	2 (mM)
3	1	reservoir	ammonium sulfate	1.7 (M)
4	1	reservoir	Tris-HCl	0.1 (M)	pH7.5
5	1	reservoir		10 (mM)