1RVE

THE CRYSTAL STRUCTURE OF ECORV ENDONUCLEASE AND OF ITS COMPLEXES WITH COGNATE AND NON-COGNATE DNA FRAGMENTS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	58.200, 71.700, 130.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.185
Rwork	0.185
RMSD bond length	0.017
RMSD bond angle	2.070
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.069 *
Total number of observations	86437 *
Number of reflections	16509 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-8 (mg/ml)
2	1	drop	PEG4000	21-24 (%(w/v))
3	1	drop		0.1-0.15 (M)
4	1	reservoir	PEG4000	10 (%)
5	1	reservoir		0.18 (M)