1RVC
MG2+ BINDING TO THE ACTIVE SITE OF ECO RV ENDONUCLEASE: A CRYSTALLOGRAPHIC STUDY OF COMPLEXES WITH SUBSTRATE AND PRODUCT DNA AT 2 ANGSTROMS RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.290, 50.340, 63.880 |
| Unit cell angles | 96.66, 108.76, 108.41 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.156 |
| Rwork | 0.156 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.830 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 * | |
| High resolution limit [Å] | 2.100 * | 2.100 * |
| Rmerge | 0.043 * | |
| Total number of observations | 39654 * | |
| Number of reflections | 26657 * | |
| Completeness [%] | 85.1 * | 57.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | EcoRV | 15 (mg/ml) | |
| 2 | 1 | 1 | phosphate | 10 (mM) | |
| 3 | 1 | 1 | 250 (mM) | ||
| 4 | 1 | 1 | EDTA | 1 (mM) | |
| 5 | 1 | 1 | DTT | 0.1 (mM) | |
| 6 | 1 | 1 | oligodeoxynucleotide | 3.0 (mg/ml) | |
| 7 | 1 | 1 | cacodylate | 10 (mM) | |
| 8 | 1 | 1 | PEG4000 | 0.8-1.4 (%) | final concentrayions |
