1RV7
Crystal structures of a Multidrug-Resistant HIV-1 Protease Reveal an Expanded Active Site Cavity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-03 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5148 |
| Spacegroup name | P 41 |
| Unit cell lengths | 44.975, 44.975, 103.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.271 |
| Rwork | 0.271 |
| R-free | 0.35000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.087 |
| RMSD bond angle | 4.885 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 5316 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 295 | MES, sodium chloride, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
