1RUT
Complex of LMO4 LIM domains 1 and 2 with the ldb1 LID domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08, 1.282, 1.170, 1.283 |
| Spacegroup name | P 3 1 2 |
| Unit cell lengths | 61.344, 61.344, 93.159 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.130 - 1.300 |
| R-factor | 0.15686 |
| Rwork | 0.155 |
| R-free | 0.19316 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.242 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.040 | 0.291 |
| Number of reflections | 45441 | |
| <I/σ(I)> | 18 | 2.4 |
| Completeness [%] | 91.3 | 79.2 |
| Redundancy | 2.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 1.0M ammonium sulfate, 0.1M MES, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
