1RUO

CATABOLITE GENE ACTIVATOR PROTEIN (CAP) MUTANT/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	258
Detector technology	IMAGE PLATE
Collection date	1993-09-01
Detector	FUJI
Spacegroup name	C 2 2 21
Unit cell lengths	137.290, 153.100, 76.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.213 *
Rwork	0.213
RMSD bond length	0.017 *
RMSD bond angle	2.420 *
Data reduction software	PURDUE (DATA PROCESSING PACKAGE)
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.700
Rmerge	0.105 *
Total number of observations	52283 *
Number of reflections	18147
Completeness [%]	83.7
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	4 *	pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	CAP	0.1-0.2 (mM)
10	1	reservoir	cAMP	2 (mM)
11	1	reservoir	n-octyl-beta-D-glucopyranoside	0.3 (%(w/v))
12	1	reservoir	PRG3350	6.5 (%(w/v))
13	1	drop	well buffer	50 (%)
2	1	drop	DNA molecule 31-2E	(%(v/v))	1.2- to 1.5-molar excess
3	1	reservoir	Mes-NaOH	50 (mM)
4	1	reservoir		200 (mM)
5	1	reservoir		100 (mM)
6	1	reservoir		100 (mM)
7	1	reservoir		0.02 (%(w/v))
8	1	reservoir	dithiothreitol	2 (mM)
9	1	reservoir	spermine	2 (mM)