1RUF

RHINOVIRUS 14 (HRV14) (MUTANT WITH ASN 1 219 REPLACED BY ALA (N219A IN CHAIN 1)

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Detector technology	FILM
Collection date	1990-09
Detector	KODAK
Wavelength(s)	1.563
Spacegroup name	P 21 3
Unit cell lengths	445.100, 445.100, 445.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.900
Data reduction software	PURDUE (DATA PROCESSING PACKAGE)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.900
Rmerge	0.118
Total number of observations	72027 *
Number of reflections	60802
Completeness [%]	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	virus	5 (mg/ml)
2	1	drop	PEG8000	0.125 (%(w/v))	or 0.25 %(w/v)
3	1	drop	Tris-HCl	0.01 (M)
4	1	reservoir	PEG8000	0.25 (%(w/v))	or 0.5%(w/v)
5	1	reservoir		0.02 (M)
6	1	reservoir	Tris-HCl	0.01 (M)