1RU4
Crystal structure of pectate lyase Pel9A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.8700 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.330, 57.830, 114.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.000 - 1.600 |
| R-factor | 0.177 |
| Rwork | 0.167 |
| R-free | 0.18600 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.075 | 0.124 |
| Number of reflections | 49017 | |
| Completeness [%] | 99.1 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | 12% PEG 3350,100mM sodium chloride, 400mM citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
