1RTX
Crystal Structure of Synechocystis Hemoglobin with a Covalent Heme Linkage
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL1-5 |
Synchrotron site | SSRL |
Beamline | BL1-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-03 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.737570, 1.239793, 1.739925 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.500, 46.000, 61.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.760 - 1.800 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.22400 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 3.956 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.760 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.211 |
Number of reflections | 10617 | |
Completeness [%] | 97.2 | 78.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 30% PEG MME 5000, 0.2M ammonium sulfate, 0.01 cadmium chloride, 0.1M MES, 0.01M potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 30% PEG MME 5000, 0.2M ammonium sulfate, 0.01 cadmium chloride, 0.1M MES, 0.01M potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 30% PEG MME 5000, 0.2M ammonium sulfate, 0.01 cadmium chloride, 0.1M MES, 0.01M potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |