1RTR
Crystal Structure of S. Aureus Farnesyl Pyrophosphate Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 97 |
| Detector technology | CCD |
| Collection date | 2002-11-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 150.201, 150.201, 163.184 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.22662 |
| Rwork | 0.222 |
| R-free | 0.26573 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.211 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 38076 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 97.0 | 0.93 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Nanoliter Sitting Drop Vapour Diffusion | 5 | 298 | PEG 1000, Phosphate, pH 5, Nanoliter Sitting Drop Vapour Diffusion, temperature 298K |
