1RSU
COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG II PEPTIDE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06-13 |
| Detector | RIGAKU |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 58.400, 58.400, 176.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.21300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 26.755 * |
| Data reduction software | R-AXIS (IIC) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.380 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.092 |
| Number of reflections | 16909 |
| Completeness [%] | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein solution | 0.002ml | |
| 2 | 1 | reservoir | ammonium sulfate | 0.8 (M) | |
| 3 | 1 | reservoir | sodium phosphate | 100 (mM) |
