1RST

COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG PEPTIDE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-12-08
Detector	RIGAKU
Spacegroup name	I 41 2 2
Unit cell lengths	58.300, 58.300, 176.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.189
Rwork	0.189
R-free	0.22700
RMSD bond length	0.012
RMSD bond angle	27.963 *
Data reduction software	R-AXIS (IIC)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.380
High resolution limit [Å]	1.700
Rmerge	0.089
Number of reflections	16059
Completeness [%]	92.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.6 (M)
2	1	reservoir	sodium phosphate	100 (mM)