1RRL
Soybean Lipoxygenase (LOX-3) at 93K at 2.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-15 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.400, 133.500, 60.700 |
| Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.090 |
| R-factor | 0.212 |
| Rwork | 0.207 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1no3 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.207 |
| Data reduction software | bioteX |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.170 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 65033 | |
| <I/σ(I)> | 8.1 | 1.4 |
| Completeness [%] | 64.5 | 0.4 |
| Redundancy | 2 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 295 | 20% PEG 8000, citrate-phosphate buffer 0.05M, tris.HCl, 0.2% sodium azide, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 295K, pH 5.30 |
