1RRH
Soybean Lipoxygenase (LOX-3) at ambient temperatures at 2.0 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-15 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.709, 137.211, 61.792 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.18155 |
| Rwork | 0.179 |
| R-free | 0.23059 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1no3 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.878 |
| Data reduction software | bioteX |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 | 0.445 |
| Number of reflections | 54258 | |
| <I/σ(I)> | 13.5 | 1.47 |
| Completeness [%] | 86.2 | 0.767 |
| Redundancy | 2 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 295 | 20% PEG 8000, citrate-phosphate buffer 0.05M, tris.HCl, 0.2% sodium azide, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
