1RRF

NON-MYRISTOYLATED RAT ADP-RIBOSYLATION FACTOR-1 COMPLEXED WITH GDP, MONOMERIC CRYSTAL FORM

Experimental procedure

Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	69.700, 45.250, 60.450
Unit cell angles	90.00, 109.60, 90.00

Refinement procedure

Resolution	8.000 - 3.000
R-factor	0.231
Rwork	0.231
R-free	0.31900
RMSD bond length	0.025
RMSD bond angle	3.341 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	21.000
High resolution limit [Å]	3.000
Rmerge	0.080
Total number of observations	7676 *
Number of reflections	3415
Completeness [%]	94.1
Redundancy	2.25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.29 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium citrate	0.05 (M)
3	1	drop	2-propanol	10 (%(v/v))
4	1	drop	PEG4000	10 (%)
5	1	reservoir	sodium citrate	0.1 (M)
6	1	reservoir	2-propanol	20 (%(v/v))
7	1	reservoir	PEG4000	20 (%)