1RRE

Crystal structure of E.coli Lon proteolytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 31
Unit cell lengths	86.370, 86.370, 124.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.960 - 1.750
R-factor	0.21025
Rwork	0.205
R-free	0.26269
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.750
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.075
Number of reflections	105429
<I/σ(I)>	19.2	2.1
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	ammonium sulfate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K