1RR7
Crystal structure of the Middle Operon Regulator protein of Bacteriophage Mu
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0721 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.631, 81.631, 44.843 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| Rwork | 0.252 |
| R-free | 0.26800 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 11.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 7746 |
| <I/σ(I)> | 48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | Sodium chloride, guanidine hydrocholride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | Sodium chloride, guanidine hydrocholride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
