1RR7
Crystal structure of the Middle Operon Regulator protein of Bacteriophage Mu
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0721 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 81.631, 81.631, 44.843 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
Rwork | 0.252 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 11.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 7746 |
<I/σ(I)> | 48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | Sodium chloride, guanidine hydrocholride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | Sodium chloride, guanidine hydrocholride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |