1RQG
Methionyl-tRNA synthetase from Pyrococcus abyssi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 113.150, 113.150, 287.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.500 - 2.900 |
| R-factor | 0.2436 |
| Rwork | 0.244 |
| R-free | 0.29680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a8h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.500 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 20834 | |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | 3.5% peg6000, 2% dioxane, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
