1RQF
Structure of CK2 beta subunit crystallized in the presence of a p21WAF1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-03-05 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9340 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 145.422, 170.626, 74.546 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 11.970 - 2.890 |
R-factor | 0.24 |
Rwork | 0.238 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qf8 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 11.970 | 3.080 |
High resolution limit [Å] | 2.890 | 2.890 |
Rmerge | 0.067 | 0.336 |
Number of reflections | 41256 | |
<I/σ(I)> | 7.2 | 2.2 |
Completeness [%] | 99.3 | 99.8 |
Redundancy | 4.2 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 295 | PEG 4000, bicine, magnesium chloride, DTT, isopropanol, pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |