1RQB
Propionibacterium shermanii transcarboxylase 5S subunit
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2003-03-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0734, 0.9803, 0.9808 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.464, 145.938, 79.008 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.120 - 1.900 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.19100 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.164 |
| Number of reflections | 42109 | |
| <I/σ(I)> | 19.5 | 6.5 |
| Completeness [%] | 95.2 | 87 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG 4000, Tris, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
