1RPX
D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM SOLANUM TUBEROSUM CHLOROPLASTS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-01 |
| Detector | SIEMENS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 146.100, 146.100, 93.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 2.300 |
| R-factor | 0.174 * |
| Rwork | 0.174 |
| R-free | 0.21200 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 25.300 * |
| Data reduction software | Agrovata |
| Data scaling software | XDS |
| Refinement software | X-PLOR (3.8.5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.060 * | 0.206 * |
| Total number of observations | 265449 * | |
| Number of reflections | 49783 | |
| <I/σ(I)> | 11.6 | 3.6 |
| Completeness [%] | 97.2 | 87.1 |
| Redundancy | 5.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 20 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.5 (M) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | MOPS/NaOH | 10 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | 1 (mM) | ||
| 7 | 1 | reservoir | ammonium sulfate | 2.8 (M) |
