1RPL

2.3 ANGSTROMS CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF DNA POLYMERASE BETA

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	120.800, 63.400, 38.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.22
Rwork	0.220
RMSD bond length	0.021
RMSD bond angle	3.500
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.042 *
Total number of observations	37975 *
Number of reflections	13365 *
Completeness [%]	97.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	drop	HEPES	50 (mM)
3	1	drop		50 (mM)
4	1	drop	dithiothreitol	0.1 (mM)
5	1	reservoir	PEG1500	12-14 (%)
6	1	reservoir	HEPES	100 (mM)