1RP0
Crystal Structure of Thi1 protein from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.28269, 0.915013 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 102.356, 133.147, 142.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.710 - 1.600 |
| R-factor | 0.14072 |
| Rwork | 0.139 |
| R-free | 0.17063 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.915 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.710 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 63420 | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 99.4 | 93.6 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | TES, magnesium chloride, MES, MPD, heptane-1,2,3-triol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
