1ROV
Lipoxygenase-3 Treated with Cumene Hydroperoxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2003-06-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.001, 136.901, 61.512 |
| Unit cell angles | 90.00, 96.24, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.22130 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.034 |
| RMSD bond angle | 27.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 * | |
| Total number of observations | 109207 * | |
| Number of reflections | 50653 * | |
| <I/σ(I)> | 15.9 | 3.3 |
| Completeness [%] | 83.0 * | 75.5 |
| Redundancy | 2.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * | 298 | PEG 8000,sodium citrate-phosphate, sodium phosphate , pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 0.1 (M) | pH7.0 |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | PEG8000 | 20 (%(v/v)) | |
| 4 | 1 | reservoir | sodium citrate phosphate | 0.05 (M) | pH4.6 |
| 5 | 1 | reservoir | sodium phosphate | 0.1 (M) | pH7.0 |
