1RO2
Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1- Triple mutant F50M/L107M/L110M manganese soak
Experimental procedure
| Experimental method | MULTIPLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.005, 0.9793, 1.280, 0.9797 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 45.811, 119.907, 41.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| Rwork | 0.252 |
| R-free | 0.26500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.630 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.062 | 0.234 |
| Number of reflections | 31761 | 2752 * |
| Completeness [%] | 98.3 | 86.6 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | Tris, sodium acetate, PEG 4000, DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-Cl | 100 (mM) | pH8.5 |
| 2 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 30 (%(w/v)) | |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 6 | 1 | drop | 150 (mM) | ||
| 7 | 1 | drop | dithiothreitol | 3 (mM) | |
| 8 | 1 | drop | protein | 25 (mg/ml) |
