1RNQ

RIBONUCLEASE A CRYSTALLIZED FROM 8M SODIUM FORMATE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	290
Detector technology	AREA DETECTOR
Collection date	1993
Detector	SIEMENS
Spacegroup name	P 32 2 1
Unit cell lengths	64.936, 64.936, 65.289
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.161
Rwork	0.161
RMSD bond length	0.012
RMSD bond angle	0.033 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.800	2.090
High resolution limit [Å]	2.000	2.000
Rmerge	0.036	0.121
Number of reflections	9461
<I/σ(I)>	28.5	4.31
Completeness [%]	86.8	64.8
Redundancy	2.61	1.55

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	reservoir	sodium formate	3 (M)
3	1	reservoir		3 (M)
4	1	reservoir	sodium acetate	0.1 (M)